CD105 Antibody / Endoglin Cross-Species Detection Antibody (Rabbit Polyclonal) (R32935)

CD105 Antibody Mouse Kidney IHC. Immunohistochemistry analysis of FFPE mouse kidney tissue using CD105 Antibody demonstrates HRP-DAB brown membranous staining in endothelial cells lining renal vasculature, including vessels associated with glomerular and interstitial regions, consistent with Endoglin / CD105 expression as a vascular marker and supporting cross-species detection in mouse tissue, while surrounding tubular epithelial cells show minimal staining; nuclei are counterstained blue. HIER: steam tissue sections in pH 6 citrate buffer for 20 min and allow to cool prior to testing.

CD105 Antibody Mouse Kidney Tissue IHC. IHC staining of FFPE mouse kidney tissue using CD105 antibody at 2 ug/ml. Heat-induced epitope retrieval was performed in pH8 EDTA buffer before incubation with the primary antibody, followed by an HRP-conjugated secondary antibody and DAB chromogen. Distinct membranous staining is observed in vascular endothelial cells throughout the kidney, with little staining of the surrounding renal parenchyma, consistent with the established endothelial expression pattern of Endoglin (CD105). This result demonstrates that CD105 antibody, an Endoglin Cross-Species Detection antibody, is suitable for immunohistochemical detection of endothelial cells in mouse tissue.

CD105 Antibody Rat Kidney IHC. Immunohistochemistry analysis of FFPE rat kidney tissue using CD105 Antibody demonstrates HRP-DAB brown membranous staining in endothelial cells lining glomerular and peritubular capillary structures, consistent with Endoglin / CD105 expression as an endothelial-associated protein, supporting cross-species detection in rat tissue; nuclei are counterstained blue. HIER: steam sections in pH 6 citrate buffer for 20 min and allow to cool prior to testing.

CD105 Antibody Mouse and Rat Kidney WB. Western blot analysis of rat and mouse kidney lysates using CD105 antibody. Lane 1: rat kidney. Lane 2: mouse kidney. A predominant immunoreactive band is detected at approximately 95 kDa in both tissue lysates, with an additional lower-molecular-weight band visible in the mouse sample. Although the predicted molecular weight of CD105 (Endoglin) is approximately 70 kDa, the protein commonly migrates at a higher apparent molecular weight due to extensive N-linked glycosylation. These results demonstrate that CD105 antibody, an Endoglin Cross-Species Detection antibody, reliably detects endogenous CD105 in both rat and mouse kidney by Western blot.