CD105 Antibody / Endoglin (RQ7690)

Immunofluorescence analysis of CD105 in human ovarian cancer tissue. Formalin-fixed, paraffin-embedded human ovarian cancer tissue was stained using CD105 antibody (red), with nuclei counterstained using DAPI (blue). Immunoreactivity highlights endothelial cell-associated membranous staining within tumor vasculature, consistent with CD105 expression in angiogenic blood vessels. Heat-induced epitope retrieval was performed by steaming tissue sections in EDTA buffer, pH 8.0, for 20 minutes prior to immunostaining.

IHC staining of FFPE human endometrial adenocarcinoma tissue with CD105 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with CD105 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with CD105 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human bladder urothelial carcinoma tissue with CD105 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of human 1) SiHa and 2) HeLa cell lysate with CD105 antibody. Observed molecular weight: 70/90 kDa (monomer, unmodified/glycosylated); 140-180 kDa (dimer, unmodified/glycosylated).

Flow cytometry testing of human U-87 MG cells with CD105 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CD105 antibody.