CCAR2 Antibody / DBC1 (RQ6544)

IHC staining of FFPE human renal clear cell carcinoma tissue with CCAR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human pancreatic carcinoma tissue with CCAR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer tissue with CCAR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human gall bladder adenosquamous carcinoma tissue with CCAR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with CCAR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human laryngeal carcinoma tissue with CCAR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse brain tissue with CCAR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat brain tissue with CCAR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat brain tissue with CCAR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human U-2 OS cells with CCAR2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human Jurkat, 2) monkey COS-7, 3) human RT4, 4) human K562, 5) human HEL, 6) rat brain, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with CCAR2 antibody. Expected molecular weight: 103-140 kDa depending on level of acetylation.

Flow cytometry testing of human A431 cells with CCAR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CCAR2 antibody.