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Home >> Antibodies >> CBX8 Antibody

CBX8 Antibody (RQ5872)

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  Catalog No Formulation Size Price (USD)  
Image RQ5872 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human rectal cancer with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human rectal cancer with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with CBX8 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human rectal cancer with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse small intestine with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat small intestine with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) A549, 3) U-2 OS, 4) COLO-320, 5) Raji, 6) SW620, 7) rat PC-3 and 8) mouse SP2/0 lysate with CBX8 antibody. Predicted molecular weight ~43 kDa.
Flow cytometry testing of human A549 cells with CBX8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CBX8 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q9HC52
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Immunocytochemistry : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This CBX8 antibody is available for research use only.
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Description

CBX8 functions as a transcriptional repressor and has a role in DNA damage response. This gene is mapped to chromosome 17q25.3 based on an alignment of the CBX8 sequence with the genomic sequence (GRCh38).

Application Notes

Optimal dilution of the CBX8 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-R389) was used as the immunogen for the CBX8 antibody.

Storage

After reconstitution, the CBX8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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