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Home >> Antibodies >> CBFB Antibody

CBFB Antibody (F54434)

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  Catalog No Formulation Size Price (USD)  
Image F54434-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54434-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of 1) human K562 and 2) mouse lung lysate with CBFB antibody. Predicted molecular weight ~22 kDa.
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Western blot testing of 1) human K562 and 2) mouse lung lysate with CBFB antibody. Predicted molecular weight ~22 kDa.
Western blot testing of human 1) Jukat and 2) Raji cell lysate with CBFB antibody. Predicted molecular weight ~22 kDa.
Immunofluorescent staining of human HEK293 cells with CBFB antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human brain tissue with CBFB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HL60 cells with CBFB antibody; Blue=isotype control, Green= CBFB antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q13951
Localization Nuclear
Applications Western blot : 1:500-1:2000
Immunofluorescence : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This CBFB antibody is available for research use only.
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Description

Core-binding factor subunit beta is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters.

Application Notes

The stated application concentrations are suggested starting points. Titration of the CBFB antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 61-90 from the human protein was used as the immunogen for the CBFB antibody.

Storage

Aliquot the CBFB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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