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Home >> Antibodies >> Caveolin-3 Antibody / CAV3

Caveolin-3 Antibody / CAV3 (RQ4913)

  Catalog No Formulation Size Price (USD)  
Image RQ4913 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human placenta, 2) U-87 MG, 3) human HeLa, 4) rat stomach, 5) rat testis, 6) mouse stomach and 7) mouse testis lysate with Caveolin-3 antibody at 0.5ug/ml. Predicted molecular weight: ~17 kDa but routinely observed at 20~25 kDa.
IHC staining of FFPE human skeletal muscle with Caveolin-3 antibody at 1ug/ml (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with Caveolin-3 antibody at 1ug/ml (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse heart with Caveolin-3 antibody at 1ug/ml (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat heart with Caveolin-3 antibody at 1ug/ml (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat heart with Caveolin-3 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P56539
Localization Cytoplasmic, plasma membrane
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Caveolin-3 antibody is available for research use only.
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Description

Caveolin-3 is a protein that in humans is encoded by the CAV3 gene. This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites.

Application Notes

Optimal dilution of the Caveolin-3 antibody should be determined by the researcher.

Immunogen

Amino acids M1-D55 from the human protein were used as the immunogen for the Caveolin-3 antibody.

Storage

After reconstitution, the Caveolin-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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