CAV2 Antibody / Caveolin-2 (RQ5696)

Immunofluorescent staining of FFPE human A549 cells with CAV2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunofluorescent staining of FFPE human intestinal cancer with CAV2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

IHC staining of FFPE human rectal cancer with CAV2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse lung with CAV2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat lung with CAV2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of human 1) HeLa, 2) A549, 3) U-2 OS, 4) K562, 5) HT1080, 6) Caco-2, 7) HEK293, 8) monkey COS-7, 9) rat skeletal muscle, 10) rat heart, 11) mouse skeletal muscle, 12) mouse heart and 13) mouse NIH 3T3 lysate with CAV2 antibody. Predicted molecular weight ~18 kDa.

Flow cytometry testing of human A549 cells with CAV2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CAV2 antibody.