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Home >> Antibodies >> Catalase Antibody

Catalase Antibody (R32390)

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  Catalog No Formulation Size Price (USD)  
Image R32390 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat skeletal muscle, 2) mouse skeletal muscle and 3) human A549 lysate with Catalase antibody. Expected/observed molecular weight ~60 kDa.
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Western blot testing of 1) rat skeletal muscle, 2) mouse skeletal muscle and 3) human A549 lysate with Catalase antibody. Expected/observed molecular weight ~60 kDa.
IHC testing of FFPE human liver cancer tissue with Catalase antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE mouse liver with Catalase antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE rat liver with Catalase antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P04040
Localization Cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This Catalase antibody is available for research use only.
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Description

Catalase is a key antioxidant enzyme in the bodies defensing against oxidative stress. It is also a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene.

Application Notes

Optimal dilution of the Catalase antibody should be determined by the researcher.

Immunogen

Amino acids E344-L527 of the human protein were used as the immunogen for the Catalase antibody.

Storage

After reconstitution, the Catalase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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