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Home >> Antibodies >> Caspase-9 Antibody

Caspase-9 Antibody (F50929)

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  Catalog No Formulation Size Price (USD)  
Image F50929-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50929-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of Caspase-9 antibody and Jurkat lysate
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Western blot analysis of Caspase-9 antibody and Jurkat lysate
IHC analysis of FFPE human hepatocarcinoma with Caspase-9 antibody
Flow cytometric analysis of MDA-231 cells using Caspase-9 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P55211
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This Caspase-9 antibody is available for research use only.
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Description

Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.

Application Notes

Titration of the Caspase-9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This Caspase-9 antibody was produced from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S196 of human CASP9.

Storage

Aliquot the Caspase-9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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