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Home >> Antibodies >> Caspase-8 Antibody (large subunit)

Caspase-8 Antibody (large subunit) (RQ6832)

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  Catalog No Formulation Size Price (USD)  
Image RQ6832 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human U-251 cells with Caspase-8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caspase-8 antibody.
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Flow cytometry testing of human U-251 cells with Caspase-8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caspase-8 antibody.
Western blot testing of human 1) HepG2, 2) Caco-2, 3) PANC-1, 4) K562 and 5) RT4 cell lysate with Caspase-8 antibody. Predicted molecular weight: ~55 kDa (pro), ~40 kDa (large + small subunit), ~20 kDa (large subunit).
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q14790
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Caspase-8 antibody is available for research use only.
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Description

CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. In addtion, this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Application Notes

Optimal dilution of the Caspase-8 antibody should be determined by the researcher.

Immunogen

Amino acids HFEIKPHDDCTVEQIYE from the human protein were used as the immunogen for the Caspase-8 antibody.

Storage

After reconstitution, the Caspase-8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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