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Home >> Antibodies >> Caspase-3 Antibody

Caspase-3 Antibody (R31602)

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Image R31602 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of Caspase-3 antibody and Lane 1: rat liver; 2: rat thymus; 3: SMMC-7721 lysate. Predicted molecular weight ~32 kDa.
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Western blot testing of Caspase-3 antibody and Lane 1: rat liver; 2: rat thymus; 3: SMMC-7721 lysate. Predicted molecular weight ~32 kDa.
IHC-P: Caspase-3 antibody testing of human lung cancer tissue.
IHC-P: Caspase-3 antibody testing of mouse intestine tissue.
IHC-P: Caspase-3 antibody testing of rat intestine tissue.
Western blot testing of Caspase-3 antibody and recombinant human protein (0.5ng).
Flow cytometry testing of human K562 cells with Caspase-3 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Caspase-3 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 836
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Flow Cytometry : 1-3ug/10^6 cells
Limitations This Caspase-3 antibody is available for research use only.
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Description

Caspase-3 is an enzyme which interacts with Survivin, XIAP, CFLAR, CASP8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located on 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. They exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. CASP3 is the predominant cysteine-aspartic acid protease involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimers disease. And protein activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the proteins influence on bone mineral density should be considered in any in vivo application of Caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 67-175) was used as the immunogen for this Caspase-3 antibody. This amino acid sequence is from the large subunit.

Storage

After reconstitution, the Caspase-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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