• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> Caspase-3 Antibody

Caspase-3 Antibody (R31602)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image R31602 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
Bulk quote request
Western blot testing of 1) lysate from untreated human Jurkat cells, 2) lysate from Jurkat cells treated with 1 uM staurosporine, 3) lysate from Jurkat cells treated with 2 uM staurosporine and 4) lysate from untreated human HepG2 cell lysate with Caspase-3 antibody.
›
Western blot testing of 1) lysate from untreated human Jurkat cells, 2) lysate from Jurkat cells treated with 1 uM staurosporine, 3) lysate from Jurkat cells treated with 2 uM staurosporine and 4) lysate from untreated human HepG2 cell lysate with Caspase-3 antibody.
Western blot testing of 1) lysate from untreated human Jurkat cells, 2) lysate from Jurkat cells treated with 1 uM staurosporine and untreated 3) rat thymus, 4) rat C6, 5) mouse NIH 3T3, 6) mouse RAW264.7 and 7) mouse ANA-1 cell lysate with Caspase-3 antibody.
IHC staining of FFPE human tonsil tissue with Caspase-3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with Caspase-3 antibody (green).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Gene ID 836
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Limitations This Caspase-3 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

  • Applications : WB, IHC
    Reactivity : Human
    Recrabbitmono
  • Applications : WB, IHC-P
    Reactivity : Human, Mouse, Rat
  • Applications : WB, ELISA
    Reactivity : Human
  • Applications : WB, IHC-P, FACS
    Reactivity : Human
  • Applications : WB, IHC-P, IF, FACS
    Reactivity : Human
  • Applications : WB, IHC-P
    Reactivity : Human, Mouse, Rat
    Rab Mono Image
  • Applications : WB
    Reactivity : Human, Mouse, Rat
  • Applications : WB, IHC-P, ICC
    Reactivity : Human
  • Applications : WB, IHC-P
    Reactivity : Mouse, Rat
  • Applications : WB, IHC, FACS, ELISA
    Reactivity : Human
    Pred. Reactivity : Mouse, Rat, Primate, Rabbit
  • Applications : WB, IHC, FACS, ELISA
    Reactivity : Human
  • Applications : WB, FACS, IHC, ELISA
    Reactivity : Human
  • Applications : DB, ELISA
    Reactivity : Mouse
  • Applications : DB, ELISA
    Reactivity : Mouse
  • Applications : WB, ELISA (peptide)
    Reactivity : Human
    Pred. Reactivity : Dog, Mouse, Rat
  • Applications : WB, IF, FACS
    Reactivity : Human

Description

Caspase-3 is an enzyme which interacts with Survivin, XIAP, CFLAR, CASP8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located on 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. They exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. CASP3 is the predominant cysteine-aspartic acid protease involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimers disease. And protein activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the proteins influence on bone mineral density should be considered in any in vivo application of Caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 67-175) was used as the immunogen for this Caspase-3 antibody. This amino acid sequence is from the large subunit.

Storage

After reconstitution, the Caspase-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all Caspase-3 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.