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Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Titration of the Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Purified His-tagged protein was used to produced this monoclonal Caspase-3 antibody.
Aliquot the Caspase-3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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