Carbonic Anhydrase XII Antibody / CA12 Polyclonal Antibody (FY13510)

Carbonic Anhydrase XII Antibody by IHC in rat kidney. Immunohistochemistry of FFPE rat kidney tissue using Carbonic Anhydrase XII Antibody at 2 ug/ml. Strong brown immunoreactivity is observed along the membranes of selected renal tubular epithelial cells, with comparatively weak staining in surrounding structures. The well-defined tubular architecture and localized staining pattern are consistent with the membrane-associated distribution of CA12 in renal tissue. Hematoxylin counterstaining highlights blue-purple nuclei. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0, followed by overnight primary antibody incubation at 4oC. This CA12 Polyclonal Antibody demonstrates prominent tubular epithelial immunoreactivity.

Carbonic Anhydrase XII Antibody by IHC in human breast cancer with membranous staining. Immunohistochemistry of FFPE human breast cancer tissue using Carbonic Anhydrase XII Antibody at 2 ug/ml. Brown immunoreactivity is distributed along the membranes and cytoplasm of numerous tumor-associated cells, with distinct cellular boundaries and relatively limited background staining. Hematoxylin counterstaining identifies blue-purple nuclei throughout the section. Heat-mediated antigen retrieval was performed in EDTA buffer at pH 8.0, followed by overnight primary antibody incubation at 4oC. This CA12 Polyclonal Antibody demonstrates membrane-associated immunoreactivity consistent with the localization of carbonic anhydrase XII.

Carbonic Anhydrase XII Antibody by IHC in human breast cancer with heterogeneous tumor staining. Immunohistochemistry of FFPE human breast cancer tissue using Carbonic Anhydrase XII Antibody at 2 ug/ml. Moderate-to-strong brown immunoreactivity is observed in tumor-associated cells, with predominantly membranous and cytoplasmic staining. Signal intensity varies among neighboring cells, producing a heterogeneous distribution. Hematoxylin counterstaining highlights blue-purple nuclei. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0, followed by overnight incubation at 4oC. This CA12 Polyclonal Antibody demonstrates immunoreactivity within the cellular components of breast cancer tissue.

Carbonic Anhydrase XII Antibody by IF in A549 cells. Immunofluorescence staining of human A549 cells using Carbonic Anhydrase XII Antibody at 5 ug/ml. Red Cy3 fluorescence highlights intracellular structures and portions of the cell periphery, indicating a predominantly cytoplasmic and partially membrane-associated staining pattern. Nuclei are counterstained blue with DAPI. Enzymatic antigen retrieval was performed for 15 minutes, followed by overnight primary antibody incubation at 4oC. Cy3-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1:500. This CA12 Polyclonal Antibody demonstrates immunofluorescent detection of carbonic anhydrase XII in cultured cells.

Carbonic Anhydrase XII Antibody by WB in A549, RT4, MCF-7 and U2OS cells. Western blot analysis of human whole-cell lysates using rabbit polyclonal Carbonic Anhydrase XII Antibody. Lane 1: A549; lane 2: RT4; lane 3: MCF-7; lane 4: U2OS. Samples were separated by 10% SDS-PAGE under reducing conditions, with 30 ug of protein loaded per lane. A prominent band is detected at approximately 45 kDa in all four samples, compared with the calculated CA12 molecular weight of 39.5 kDa. The slightly higher apparent molecular weight is consistent with glycosylation of this membrane-associated protein, although this has not been experimentally confirmed. The CA12 Polyclonal Antibody was used at 0.5 ug/ml.