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Home >> Antibodies >> Carbonic Anhydrase I Antibody / CA1

Carbonic Anhydrase I Antibody / CA1 [clone 4D5] (RQ6239)

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Image RQ6239 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human SiHa cells with Carbonic Anhydrase I antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Carbonic Anhydrase I antibody.
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Flow cytometry testing of human SiHa cells with Carbonic Anhydrase I antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Carbonic Anhydrase I antibody.
IHC staining of FFPE human placenta with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma with Carbonic Anhydrase I antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human K562, 2) rat spleen and 3) mouse spleen lysate with Carbonic Anhydrase I antibody. Predicted molecular weight: ~29 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 4D5
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P00915
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Carbonic Anhydrase I antibody is available for research use only.
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Description

Carbonic anhydrase 1 is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participates in a variety of biologic processes like respiration, calcification, acid-base balance etc.

Application Notes

Optimal dilution of the Carbonic Anhydrase I antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids D9-F261) was used as the immunogen for the Carbonic Anhydrase I antibody.

Storage

After reconstitution, the Carbonic Anhydrase I antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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