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Home >> Antibodies >> Carbonic Anhydrase I Antibody

Carbonic Anhydrase I Antibody (R31859)

  Catalog No Formulation Size Price (USD)  
Image R31859 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of rat 1) spleen, 2) liver, 3) kidney and 4) human placenta lysate with Carbonic Anhydrase I antibody. Predicted/observed molecular weight: ~29 kDa.
IHC testing of FFPE human liver cancer tissue with Carbonic Anhydrase I antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P00915
Localization Cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This Carbonic Anhydrase I antibody is available for research use only.
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Description

Carbonic anhydrase I is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participates in a variety of biologic processes like respiration, calcification, acid-base balance etc.

Application Notes

Optimal dilution of the Carbonic Anhydrase I antibody should be determined by the researcher.

Immunogen

Amino acids 9-261 of human CA1 were used as the immunogen for the Carbonic Anhydrase I antibody.

Storage

After reconstitution, the Carbonic Anhydrase I antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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