CAMK II alpha Antibody / Calcium Signaling Kinase Antibody (RQ5962)

CAMK II alpha Antibody MCF7 Cell IF. Immunofluorescent staining of MCF7 cells with CAMK II alpha antibody (green). Heat-induced epitope retrieval (HIER) was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. Nuclei are counterstained with DAPI (blue). CAMK II alpha immunoreactivity is observed predominantly within the cytoplasm, consistent with the expected localization of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2A), a calcium signaling kinase that regulates calcium-dependent signal transduction, protein phosphorylation, and intracellular signaling pathways.

CAMK II alpha Antibody Rat and Mouse Brain WB. Western blot testing of different lots of 1-2) rat brain and 3-4) mouse brain tissue lysates with CAMK II alpha antibody. A prominent immunoreactive band is detected at approximately 54 kDa in all lysates, consistent with the predicted molecular weight of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2A). An additional band at approximately 65 kDa is consistently observed and likely represents a phosphorylated or other post-translationally modified form of this calcium signaling kinase, which plays essential roles in synaptic plasticity, learning, memory, and neuronal signal transduction.

CAMK II alpha Antibody U-87 MG Cell FACS. Flow cytometric analysis of human U-87 MG cells stained with CAMK II alpha antibody at 1 ug/million cells following goat serum blocking. Red: cells alone. Green: isotype control. Blue: CAMK II alpha antibody. The pronounced rightward shift of the antibody-stained population relative to the isotype control demonstrates specific detection of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2A), a calcium signaling kinase that regulates calcium-dependent signal transduction, synaptic plasticity, protein phosphorylation, and neuronal signaling pathways.

CAMK II alpha Antibody ANA-1 Cell FACS. Flow cytometric analysis of mouse ANA-1 cells stained with CAMK II alpha antibody at 1 ug/million cells following goat serum blocking. Red: cells alone. Green: isotype control. Blue: CAMK II alpha antibody. The rightward shift of the antibody-stained population relative to the isotype control demonstrates specific detection of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2A), a calcium signaling kinase that regulates calcium-dependent signal transduction, protein phosphorylation, synaptic plasticity, and neuronal signaling pathways.

CAMK II alpha Antibody NRK Cell FACS. Flow cytometric analysis of rat NRK cells stained with CAMK II alpha antibody at 1 ug/million cells following goat serum blocking. Red: cells alone. Green: isotype control. Blue: CAMK II alpha antibody. The pronounced rightward shift of the antibody-stained population relative to the isotype control demonstrates specific detection of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2A), a calcium signaling kinase that regulates calcium-dependent signal transduction, protein phosphorylation, synaptic plasticity, and downstream cellular signaling pathways.

CAMK II alpha Antibody Rat Brain IF. Immunofluorescent staining of FFPE rat brain tissue with CAMK II alpha antibody (green). Heat-induced epitope retrieval (HIER) was performed by steaming sections in pH 8 EDTA buffer for 20 minutes prior to staining. Nuclei are counterstained with DAPI (blue). CAMK II alpha immunoreactivity is observed in neuronal cell bodies and processes, consistent with the expected distribution of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2A), a calcium signaling kinase that regulates synaptic plasticity, protein phosphorylation, learning, memory, and neuronal signal transduction.