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Home >> Antibodies >> Calponin Antibody

Calponin Antibody (R31348)

  Catalog No Formulation Size Price (USD)  
Image R31348 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of Calponin antibody and human 1) HeLa, 2) Jurkat, and 3) MCF7 cell lysate. Expected molecular weight ~33 kDa.
IHC-P: Calponin antibody testing of human placental tissue. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P51911
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This Calponin antibody is available for research use only.
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Description

Calponin is a basic ~34-kD protein first isolated from chicken gizzard and bovine aorta. This gene is mapped to 19p13.2. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Calponin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human Calponin (EPAHNHHAHNYYNSA) was used as the immunogen for this Calponin antibody.

Storage

After reconstitution, the Calponin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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