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Home >> Antibodies >> Calponin Antibody

Calponin Antibody [clone CLPN5-1] (V7050)

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  Catalog No Formulation Size Price (USD)  
Image V7050-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7050-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7050SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7050IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Immunofluorescent staining of PFA-fixed human K562 cells with Calponin antibody (green, clone CLPN5-1) and Reddot nuclear stain (red).
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Immunofluorescent staining of PFA-fixed human K562 cells with Calponin antibody (green, clone CLPN5-1) and Reddot nuclear stain (red).
IHC testing of FFPE human uterus and Calponin antibody (clone CLPN5-1). Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat uterus and Calponin antibody (clone CLPN5-1). Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CLPN5-1
Purity Protein G affinity chromatography
UniProt P51911
Localization Cytoplasmic
Applications Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This Calponin antibody is available for research use only.
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Description

Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Application Notes

Optimal dilution of the Calponin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the Calponin antibody.

Storage

Store the Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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