Calponin Antibody [clone CALP] (V2463)

Description

CNN1, also known as Calponin 1, is a smooth muscle-associated protein involved in regulating actin filament dynamics and contractile function. Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

This Calponin antibody complements a related Calponin 1 antibody targeting CNN1 and smooth muscle-associated contractile protein biology.

Application Notes

Optimal dilution of the Calponin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min

Immunogen

Crude human uterus extract was used as the immunogen for the Calponin antibody.

Storage

Store the Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).