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Home >> Antibodies >> Calnexin Antibody

Calnexin Antibody [clone CANX/1543] (V3795)

  Catalog No Formulation Size Price (USD)  
Image V3795-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3795-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3795SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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IHC testing of FFPE human renal cell carcinoma with Calnexin antibody (clone CANX/1543). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) PANC-1 and 2) MCF7 cell lysate with Calnexin antibody (clone CANX/1543). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
Western blot testing of human kidney lysate with Calnexin antibody (clone CANX/1543). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Calnexin antibody (clone CANX/1543). These results demonstrate the foremost specificity of the CANX/1543 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Calnexin antibody (clone CANX/1543) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CANX/1543
Purity Protein G affinity chromatography
UniProt P27824
Localization Cytoplasmic
Applications ELISA : 2-4ug/ml (order BSA/azide-free format)
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Calnexin antibody is available for research use only.
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Description

It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Application Notes

Titering of the Calnexin antibody may be required for optimal performance.

Immunogen

A portion of amino acids 1-300 from the human protein was used as the immunogen for the Calnexin antibody.

Storage

Store the Calnexin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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