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Home >> Antibodies >> Calnexin Antibody

Calnexin Antibody [clone CANX/1541] (V3199)

  Catalog No Formulation Size Price (USD)  
Image V3199-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3199-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3199SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human small intestine carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min.
IHC testing of FFPE human renal cell carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min.
IHC testing of FFPE human renal cell carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min.
Western blot testing of human kidney lysate with Calnexin antibody (clone CANX/1541). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with Calnexin antibody (clone CANX/1541). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
Immunofluorescent staining of human HeLa cells with Calnexin antibody (green) (clone CANX/1541). RedDot used as nuclear counterstain (red).
SDS-PAGE analysis of purified, BSA-free Calnexin antibody (clone CANX/1541) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name CANX/1541
Purity Protein G affinity chromatography
UniProt P27824
Localization Cytoplasmic
Applications ELISA : 2-4ug/ml (order BSA/azide-free format)
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Immunofluorescence : 1-2ug/ml
Limitations This Calnexin antibody is available for research use only.
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Description

It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Application Notes

Titering of the Calnexin antibody may be required for optimal performance.

Immunogen

A partial human recombinant protein was used as the immunogen for the Calnexin antibody.

Storage

Store the Calnexin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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