Caldesmon Antibody / CALD1 Cell Adhesion and Structural Connectivity Protein Antibody [clone RM396] (R20412)

Caldesmon Antibody / CALD1 Cell Adhesion and Structural Connectivity Protein Antibody. Western blot analysis of Caldesmon (CALD1) in human HeLa cell lysate. Lane 1: human HeLa cell lysate. A band is detected at approximately 70-80 kDa, consistent with the predicted molecular weight of Caldesmon / CALD1 and representing the lower molecular weight non-muscle isoform associated with cytoskeletal linkage and structural connectivity. CALD1 is known to produce multiple isoforms, with higher molecular weight forms typically observed at approximately 120-150 kDa in smooth muscle cells. The observed banding pattern reflects isoform-dependent expression of caldesmon in non-muscle cells, supporting its role in linking actin cytoskeleton networks to adhesion structures and maintaining structural connectivity.

Caldesmon Antibody / CALD1 Cell Adhesion and Structural Connectivity Protein Antibody. Immunohistochemistry analysis of Caldesmon (CALD1) in human colon tissue. FFPE human colon stained with Caldesmon Antibody at 1:100 demonstrates strong HRP-DAB brown cytoplasmic staining in smooth muscle cells of the muscularis layer and along the base of the mucosa. The staining highlights elongated, organized cell layers with dense filament-associated cytoplasmic signal consistent with cytoskeletal linkage at sites of structural connectivity and tissue anchoring. Epithelial cells lining the crypts show minimal staining, supporting localization of CALD1 to cytoskeletal networks that contribute to adhesion-associated structural integrity.