Caldesmon Antibody for IF / CALD1 Immunofluorescence Antibody (R32405)

Caldesmon Antibody for IF / CALD1 Immunofluorescence Antibody. Immunofluorescence analysis of Caldesmon (CALD1) in human SiHa cells. FFPE human SiHa cells stained with Caldesmon Antibody for IF / CALD1 Immunofluorescence Antibody show strong green cytoplasmic fluorescence with a filamentous, fiber-like staining pattern consistent with actin-associated cytoskeletal organization. The fluorescence signal highlights elongated cellular morphology and cytoplasmic filament networks surrounding the nuclei. Nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed using pH6 citrate buffer.

IHC testing of FFPE human intestine with Caldesmon antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.

IHC testing of FFPE mouse intestine with Caldesmon antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.

IHC testing of FFPE rat intestine with Caldesmon antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.

Western blot testing of 1) human U-2 OS, 2) human A549, 3) human HepG2, 4) human HeLa, 5) human Caco-2 and 6) mouse NIH 3T3 cell lysate with Caldesmon antibody. Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).

Flow cytometry testing of human U-87 MG cells with Caldesmon antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caldesmon antibody.