Caldesmon Antibody / CALD1 Cellular Morphology and Structural Integrity Protein Antibody (V3231)

Caldesmon Antibody / CALD1 Cellular Morphology and Structural Integrity Protein Antibody. Immunohistochemistry analysis of Caldesmon (CALD1) in human uterus tissue. FFPE human uterus stained with Caldesmon Antibody demonstrates strong HRP-DAB brown cytoplasmic staining in smooth muscle cells of the myometrium. The staining highlights elongated, spindle-shaped cells arranged in organized bundles with continuous cytoplasmic signal, consistent with preservation of cellular morphology and structural integrity supported by stable actin filament networks. Surrounding stromal and epithelial compartments show minimal staining, reinforcing the role of CALD1 in maintaining cytoskeletal structure and cell shape. Heat-induced epitope retrieval was performed using EDTA buffer at pH 7.5-8.5.

IHC testing of FFPE rat uterus with recombinant Caldesmon antibody. Required HIER: boil tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min.

Caldesmon Antibody / CALD1 Cellular Morphology and Structural Integrity Protein Antibody. Western blot analysis of Caldesmon (CALD1) in human ovary tissue lysate. Lane 1: human ovary tissue lysate. A band is detected at approximately 120-150 kDa, consistent with the predicted molecular weight of Caldesmon / CALD1 and representing the high molecular weight h-caldesmon isoform associated with structurally stable smooth muscle cells. Additional lower molecular weight bands may be observed at approximately 70-80 kDa corresponding to non-muscle isoforms that contribute to maintenance of cellular morphology and cytoskeletal integrity. The observed banding pattern reflects isoform-dependent expression of CALD1 linked to preservation of cell shape and structural organization.