- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Calbindin-D28k Antibody / CALB1 [clone MSVA-471M] (V6021)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V6021-100UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 100 ug | 559 | |
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V6021-20UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 20 ug | 259 |
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| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | MSVA-471M |
| Purity | Protein G affinity |
| UniProt | P05937 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1:50-1:100 |
| Limitations | This Calbindin-D28k/CALB1 antibody is available for research use only. |
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Calbindin-D28k antibody, also known as CALB1 antibody, recognizes Calbindin-D28k, a cytoplasmic EF-hand calcium-binding protein encoded by the CALB1 gene and widely referred to as Calbindin 1. Calbindin-D28k functions as an intracellular calcium buffer that modulates calcium-dependent signaling and protects cells from calcium overload. The protein is predominantly localized in the cytoplasm and is highly expressed in cerebellar Purkinje cells, hippocampal neurons, and subsets of cortical interneurons, making Calbindin-D28k antibody a well-established marker for defined neuronal populations.
Calbindin-D28k contains six EF-hand motifs, four of which are functional calcium-binding sites. Upon binding Ca2+, the protein undergoes conformational changes that influence intracellular signaling pathways, synaptic plasticity, and neuronal excitability. Through its buffering activity, Calbindin-D28k shapes intracellular calcium transients and contributes to neuronal resilience under metabolic and excitotoxic stress. Reduced CALB1 expression has been associated with neurodegenerative disorders including Alzheimer's disease and Parkinson's disease, where disrupted calcium homeostasis contributes to neuronal vulnerability.
In addition to its central nervous system expression, Calbindin-D28k is detected in peripheral tissues such as kidney distal tubules, intestinal epithelium, and pancreatic islet cells. In these tissues, it participates in calcium transport and secretion-related functions, supporting epithelial and endocrine physiology. Developmentally, CALB1 expression is dynamically regulated, with distinct temporal and spatial patterns observed during embryogenesis and postnatal maturation. Clone rKR6 is a recombinant mouse monoclonal antibody designed to target Calbindin-D28k in research applications. Recombinant production enables defined sequence expression rather than hybridoma expansion, supporting consistent performance between lots. This Calbindin-D28k antibody can be used to detect CALB1 expression in experimental systems focused on calcium signaling, neuronal identification, and epithelial calcium transport studies.
1. Optimal dilution of the Calbindin-D28k/CALB1 antibody should be determined by the researcher.
2. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
A recombinant fragment (around amino acids 7-96) of human Calbindin 1 protein (exact sequence is proprietary) was used as the immunogen for the Calbindin-D28k/CALB1 antibody.
Calbindin-D28k/CALB1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.
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