Calbindin Antibody / CALB1 [clone CALB1/2782] (V8170)

Immunohistochemistry analysis of Calbindin / CALB antibody (clone CALB1/2782) in human kidney tissue. Formalin-fixed, paraffin-embedded human kidney shows cytoplasmic HRP-DAB brown staining in subsets of tubular epithelial cells, while surrounding glomerular and interstitial cells are largely negative. Nuclear counterstain highlights renal architecture. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to staining.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Calbindin antibody (clone CALB1/2782). These results demonstrate the foremost specificity of the CALB1/2782 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.