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Home >> Antibodies >> Calbindin 2 Antibody / CALB2 / Calretinin

Calbindin 2 Antibody / CALB2 / Calretinin (RQ5961)

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  Catalog No Formulation Size Price (USD)  
Image RQ5961 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat brain and 2) mouse brain lysate with Calbindin 2 antibody. Expected molecular weight: ~29 kDa.
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Western blot testing of 1) rat brain and 2) mouse brain lysate with Calbindin 2 antibody. Expected molecular weight: ~29 kDa.
IHC staining of FFPE mouse brain with Calbindin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with Calbindin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with Calbindin 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human A431 cells with Calbindin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Calbindin 2 antibody.
Flow cytometry testing of human C6 cells with Calbindin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Calbindin 2 antibody.
Immunofluorescent staining of FFPE human A549 cells with Calbindin 2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P22676
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Calbindin 2 antibody is available for research use only.
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Description

Calretinin, also known as calbindin 2 (formerly 29 kDa calbindin), is a calcium-binding protein involved in calcium signaling. In humans, the calretinin protein is encoded by the CALB2 gene. It is mapped to 16q22.2. This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the Calbindin 2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids S59-Q90) was used as the immunogen for the Calbindin 2 antibody.

Storage

After reconstitution, the Calbindin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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