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Home >> Antibodies >> Cadherin 4 Antibody / CDH4 / R-Cadherin

Cadherin 4 Antibody / CDH4 / R-Cadherin (F54374)

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  Catalog No Formulation Size Price (USD)  
Image F54374-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54374-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human 1) A431 and 2) SH-SY5Y cell lysate with Cadherin 4 antibody. Expected molecular weight: 100-140 kDa depending on glycosylation level.
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Western blot testing of human 1) A431 and 2) SH-SY5Y cell lysate with Cadherin 4 antibody. Expected molecular weight: 100-140 kDa depending on glycosylation level.
Western blot testing of human HepG2 cell lysate with Cadherin 4 antibody. Expected molecular weight: 100-140 kDa depending on glycosylation level.
IHC testing of FFPE human brain tissue with Cadherin 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human brain tissue with Cadherin 4 antibody (green) and Propidium iodide (red).
Flow cytometry testing of human HepG2 cells with Cadherin 4 antibody; Blue=isotype control, Green= Cadherin 4 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P55283
Applications Immunohistochemistry (FFPE) : 1:25
Immunofluorescence : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Western blot : 1:500-1:2000
Limitations This Cadherin 4 antibody is available for research use only.
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Description

CDH4 is a classical cadherin from the cadherin superfamily. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Cadherin 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 175-203 from the human protein was used as the immunogen for the Cadherin 4 antibody.

Storage

Aliquot the Cadherin 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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