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Home >> Antibodies >> Cadherin 2 Antibody / N-Cadherin / CDH2

Cadherin 2 Antibody / N-Cadherin / CDH2 [clone CDH2/9490] (V5632)

  Catalog No Formulation Size Price (USD)  
Image V5632-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 559
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V5632-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 259
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V5632SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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Cadherin 2 Antibody Human Heart IHC. Immunohistochemistry staining of FFPE human heart tissue with Cadherin 2 antibody (clone CDH2/9490). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Cadherin 2 antibody (clone CDH2/9490) as confirmation of integrity and purity.
Cadherin 2 Antibody WB. Western blot analysis of N-Cadherin / CDH2 expression in A549 cell lysate using Cadherin 2 antibody clone CDH2/9490. Lane 1: A549 cell lysate. A prominent band is detected at approximately 120-135 kDa, consistent with the predicted molecular weight of N-Cadherin (CDH2), with the higher apparent migration reflecting known glycosylation of this transmembrane adhesion protein. Additional faint lower bands may represent proteolytic fragments or partially processed forms. The observed expression in A549 cells is consistent with N-Cadherin involvement in epithelial-to-mesenchymal transition and tumor-associated cellular remodeling.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name CDH2/9490
Purity Protein G affinity
UniProt P19022
Localization Cell surface, Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This Cadherin 2 antibody is available for research use only.
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Description

Cadherin 2 Antibody recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain.

This antibody is part of a broader antibody panel offered by NSJ Bioreagents.

Application Notes

Optimal dilution of the Cadherin 2 antibody should be determined by the researcher.

Immunogen

A recombinant human Cadherin 2 partial protein from the cytoplasmic domain was used as the immunogen for the Cadherin 2 antibody.

Storage

Aliquot the Cadherin 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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