- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Cadherin 2 Antibody / N-Cadherin Pan-Species Consistent Detection Antibody [clone CDH2/8862R] (V4537)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4537-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4537-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4537SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat, Hamster, Rabbit, Guinea pig |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | CDH2/8862R |
| Purity | Protein A/G affinity |
| UniProt | P19022 |
| Localization | Cell surface |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (Human) Western Blot : 2-4ug/ml (Human/Mouse/Rat/Hamster/Rabbit/Guinea pig) |
| Limitations | This Cadherin 2 Antibody / N-Cadherin Pan-Species Consistent Detection Antibody is available for research use only. |
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Cadherin 2 (CDH2), commonly known as N-Cadherin, is a classical calcium-dependent transmembrane adhesion protein that plays a central role in maintaining tissue architecture in both neural and cardiac systems. N-Cadherin (CDH2) is localized primarily at the plasma membrane, where it forms adherens junctions through homophilic interactions and connects to the actin cytoskeleton via catenin family proteins. The Cadherin 2 Antibody / N-Cadherin Pan-Species Consistent Detection Antibody is designed to detect this structurally conserved protein with highly uniform performance across multiple vertebrate species, making it particularly valuable for studies that require reliable comparison between experimental models.
Cadherin 2 antibody, also referred to as N-Cadherin antibody and CDH2 antibody in the literature, recognizes a highly conserved adhesion molecule expressed in both brain and heart tissues. A defining feature of this antibody is the consistent band intensity and molecular weight detection observed across human, mouse, rat, hamster, guinea pig, and rabbit samples in western blot analysis. This uniform detection profile reflects stable recognition of conserved epitopes within the N-Cadherin protein and provides a strong technical foundation for cross-species experimental design, where reproducibility across models is critical.
Structurally, N-Cadherin contains five extracellular cadherin repeat domains, a single transmembrane region, and a conserved intracellular tail that binds beta-catenin and p120-catenin to stabilize adherens junctions. These domains are under strong evolutionary constraint due to their essential role in cell-cell adhesion, which directly contributes to the highly consistent detection pattern observed across species. N-Cadherin is also glycosylated, resulting in a slightly higher apparent molecular weight on SDS-PAGE than predicted, a feature that is reproducibly observed in both neural and cardiac tissue lysates and further supports accurate target identification.
Functionally, CDH2 is critical for proper tissue organization in the nervous system, where it regulates neuronal adhesion, migration, neurite outgrowth, and synaptic stability. In cardiac tissue, N-Cadherin is a key structural component of intercalated discs, where it supports mechanical coupling between cardiomyocytes and contributes to coordinated contraction. The strong and uniform expression detected in both brain and heart tissues across species highlights the conserved biological requirement for N-Cadherin in maintaining structural integrity in highly organized, mechanically active tissues.
This Cadherin 2 antibody also demonstrates clear staining in human heart tissue by immunohistochemistry, where membranous localization highlights cardiomyocyte junctions consistent with N-Cadherin distribution at intercalated discs. The alignment between biochemical detection by western blot and tissue-level localization by immunohistochemistry strengthens confidence in target specificity and supports use in integrated experimental workflows combining protein analysis and histological evaluation.
Clone CDH2/8862R is a recombinant rabbit monoclonal antibody designed to recognize conserved regions of the N-Cadherin protein, enabling highly consistent detection across species and tissues. This pan-species consistency provides a meaningful differentiation for this Cadherin 2 antibody, particularly in studies involving translational research, comparative biology, cardiovascular disease models, and neurobiology. For researchers requiring dependable cross-species performance with minimal variability, this antibody offers a well-supported and biologically aligned solution.
This antibody is part of a broader antibody panel offered by NSJ Bioreagents.
Optimal dilution of the Cadherin 2 Antibody / N-Cadherin Pan-Species Consistent Detection Antibody should be determined by the researcher.
A recombinant partial protein sequence (within amino acids 605-905) from the human protein was used as the immunogen for the Cadherin 2 antibody.
Aliquot the Cadherin 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
N-Cadherin antibody, CDH2 antibody, Cadherin 2 WB antibody, N-Cadherin multi-species antibody, CDH2 consistency antibody
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