Cadherin 17 Antibody / Epithelial Cell Adhesion Protein Antibody [clone CDH17/2618] (V7652)

Cadherin 17 Antibody FACS. Flow cytometry analysis of human MCF7 cells using Cadherin 17 Antibody / Epithelial Cell Adhesion Protein Antibody (clone CDH17/2618) demonstrates a clear rightward shift of the antibody-stained population (blue) relative to the isotype control (red), consistent with detection of CDH17 expression. Cadherin 17 (CDH17) is a calcium-dependent cell adhesion molecule involved in epithelial tissue organization, maintenance of tissue architecture, and regulation of cell-cell interactions. CDH17 is widely studied as a marker of epithelial differentiation and gastrointestinal epithelial biology and has been investigated in multiple epithelial malignancies. Cells were stained with Cadherin 17 antibody (clone CDH17/2618) and analyzed by flow cytometry. Red = isotype control, Blue = Cadherin 17 antibody.

Cadherin 17 Antibody Colon IHC. Immunohistochemistry staining of FFPE human colon tissue using Cadherin 17 Antibody / Epithelial Cell Adhesion Protein Antibody (clone CDH17/2618) demonstrates strong membranous HRP-DAB brown staining throughout colonic epithelial cells lining glandular structures. The staining pattern is consistent with expression of CDH17, a calcium-dependent cell adhesion molecule involved in epithelial organization, maintenance of tissue architecture, and regulation of cell-cell interactions. CDH17 is highly expressed in gastrointestinal epithelium and is widely used as a marker of intestinal epithelial differentiation and epithelial lineage. HIER was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 10-20 minutes followed by cooling prior to immunostaining.

Cadherin 17 Antibody Protein Microarray Validation. Analysis of a HuProt(TM) protein microarray containing more than 19,000 full-length human proteins using Cadherin 17 Antibody / Epithelial Cell Adhesion Protein Antibody (clone CDH17/2618) demonstrates exceptional target specificity for CDH17. The antibody produced its strongest signal against CDH17, with a Z-score of 136.56 and an S-score of 129.79, indicating robust target recognition and minimal cross-reactivity with other proteins present on the array. These findings support the high specificity of clone CDH17/2618 for Cadherin 17 and reinforce its suitability for studies of epithelial differentiation, gastrointestinal biology, cell adhesion, and CDH17-associated disease pathways. Z-score represents signal strength relative to the array mean, while S-score reflects the specificity of target recognition compared with the next highest-ranking protein target.

SDS-PAGE analysis of purified, BSA-free Cadherin 17 antibody (clone CDH17/2618) as confirmation of integrity and purity.

Cadherin 17 Antibody WB. Western blot analysis of human small intestine tissue lysate using Cadherin 17 Antibody / Epithelial Cell Adhesion Protein Antibody demonstrates a strong immunoreactive band at approximately 92-100 kDa, consistent with expression of CDH17. Cadherin 17 is a calcium-dependent cell adhesion molecule predominantly expressed in gastrointestinal epithelial cells, where it contributes to epithelial organization, tissue integrity, and maintenance of cell-cell interactions. CDH17 is widely used as a marker of intestinal epithelial differentiation and gastrointestinal lineage. The observed molecular weight is consistent with the heavily glycosylated nature of Cadherin 17, which may result in migration at higher apparent molecular weights than predicted. Predicted molecular weight: approximately 92 kDa.