CDH17 Antibody / Liver-Intestine Cadherin Antibody [clone CDH17/2617] (V7651)

CDH17 Antibody WB. Western blot analysis of human small intestine lysate using CDH17 Antibody / Liver-Intestine Cadherin Antibody (clone CDH17/2617) demonstrates a strong immunoreactive band at approximately 130-140 kDa, consistent with expression of CDH17. Cadherin 17 (CDH17), also known as Liver-Intestine Cadherin (LI-cadherin), is a calcium-dependent cell adhesion molecule predominantly expressed in gastrointestinal epithelial cells, where it contributes to epithelial organization, maintenance of tissue integrity, and regulation of cell-cell interactions. The observed molecular weight is higher than the predicted size of approximately 92 kDa, a finding commonly attributed to extensive glycosylation of CDH17. These post-translational modifications frequently result in slower electrophoretic migration and increased apparent molecular weight during western blot analysis. Predicted molecular weight: approximately 92 kDa.

CDH17 Antibody Colon IHC. Immunohistochemistry staining of FFPE human colon tissue using CDH17 Antibody / Liver-Intestine Cadherin Antibody (clone CDH17/2617) demonstrates strong membranous HRP-DAB brown staining throughout colonic epithelial cells lining glandular structures. The staining pattern is consistent with expression of CDH17 (LI-cadherin), a calcium-dependent cell adhesion molecule that contributes to epithelial organization, maintenance of tissue integrity, and regulation of cell-cell interactions within the gastrointestinal tract. CDH17 is highly expressed in normal intestinal epithelium and is widely used as a marker of gastrointestinal epithelial differentiation and lineage. HIER was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 10-20 minutes followed by cooling prior to immunostaining.

CDH17 Antibody Protein Microarray Validation. Analysis of a HuProt(TM) protein microarray containing more than 19,000 full-length human proteins using CDH17 Antibody / Liver-Intestine Cadherin Antibody (clone CDH17/2617) demonstrates exceptional target specificity for CDH17. The antibody identified CDH17 as the highest-ranking target with a Z-score of 131.53 and an S-score of 111.94, indicating strong target recognition and minimal cross-reactivity with other proteins present on the array. These results support the high specificity of clone CDH17/2617 for Cadherin 17 and reinforce its suitability for studies of gastrointestinal epithelial differentiation, cell adhesion, tissue organization, and CDH17-associated disease biology. The Z-score represents signal strength relative to the mean signal across the array, while the S-score reflects target specificity by measuring the difference between the top-ranked target and the next highest-ranking protein.