Cadherin 17 Antibody Recombinant Mouse MAb / CDH17 [clone rCDH17/8512] (V5218)

Immunohistochemistry of Cadherin 17 antibody in human colon tissue. The recombinant mouse mAb clone rCDH17/8512 demonstrates strong membranous HRP-DAB brown staining along the lateral borders of colonic epithelial cells, consistent with Cadherin 17 localization in intestinal epithelium. Staining highlights well-organized glandular crypt structures with preserved epithelial polarity, while surrounding stromal tissue shows minimal background signal. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to incubation.

SDS-PAGE analysis of purified, BSA-free CDH17 antibody (clone rCDH17/8512) as confirmation of integrity and purity.

Western blot analysis of Cadherin 17 antibody in human small intestine tissue lysate. The recombinant mouse mAb clone rCDH17/8512 detects a prominent band above the predicted molecular weight of 92 kDa, consistent with Cadherin 17. Although the calculated molecular weight based on amino acid sequence is approximately 92 kDa, Cadherin 17 is heavily glycosylated, which commonly results in slower electrophoretic migration and an apparent higher molecular weight on SDS-PAGE. The observed band pattern is consistent with glycosylated CDH17 expression in small intestinal epithelial tissue.