Cadherin 16 Antibody / Kidney-Specific Expression Antibody [clone CDH16/2125] (V3691)

Cadherin 16 Antibody Human Kidney WB. Western blot analysis of Cadherin-16 / CDH16 expression in human kidney tissue lysate using Cadherin 16 antibody clone CDH16/2125. Lane 1: human kidney lysate. A band is detected at approximately 100–120 kDa, consistent with the predicted molecular weight of Cadherin-16 (CDH16), with the slightly higher apparent migration reflecting glycosylation of this kidney-restricted adhesion protein. The strong, distinct signal in kidney lysate supports the use of this antibody for kidney-specific expression analysis and aligns with the restricted distribution of CDH16 in renal epithelial tissue.

Cadherin 16 Antibody Renal Cell Carcinoma IHC. Immunohistochemical analysis of Cadherin-16 / CDH16 expression in formalin-fixed, paraffin-embedded human renal cell carcinoma using Cadherin 16 antibody clone CDH16/2125. Strong membranous staining is observed in tumor epithelial cells, highlighting well-defined cell-cell borders consistent with preserved epithelial adhesion features. The staining pattern supports kidney-specific expression and is consistent with CDH16 localization in renal tubular-derived tumor cells. Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free Cadherin 16 antibody (clone CDH16/2125) as confirmation of integrity and purity.

Cadherin 16 Antibody Microarray Specificity Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CDH16 antibody. These results demonstrate the foremost specificity of the CDH16/2125 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Cadherin 16 Antibody Kidney Tumor IHC. Immunohistochemical analysis of Cadherin-16 / CDH16 expression in formalin-fixed, paraffin-embedded human renal cell carcinoma tissue using Cadherin 16 antibody clone CDH16/2125. Distinct membranous and cytoplasmic staining is observed in carcinoma cells forming tubular and gland-like structures, while surrounding stromal regions show minimal background. The staining pattern aligns with kidney-restricted expression and supports the identification of renal epithelial origin in tumor tissue. Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Cadherin 16 Antibody Multi-Tissue WB. Western blot analysis of Cadherin-16 / CDH16 expression across human tissue lysates using Cadherin 16 antibody clone CDH16/2125. Lane 1: human brain lysate, Lane 2: human heart lysate, Lane 3: human intestine lysate, Lane 4: human kidney lysate, Lane 5: human liver lysate, Lane 6: human lung lysate, Lane 7: human pancreas lysate, Lane 8: human skeletal muscle lysate, Lane 9: human skin lysate, Lane 10: human spleen lysate, Lane 11: human stomach lysate. A band is detected at approximately 100–110 kDa exclusively in kidney lysate, consistent with the predicted molecular weight of Cadherin-16 (CDH16), supporting its role as a kidney-specific epithelial adhesion protein.

Cadherin 16 Antibody Kidney Tissue WB. Western blot analysis of Cadherin-16 / CDH16 expression in kidney tissue lysates using Cadherin 16 antibody clone CDH16/2125. Lane 1: human kidney lysate, Lane 2: mouse kidney lysate, Lane 3: rat kidney lysate. A band is detected at approximately 100–110 kDa in human kidney lysate, consistent with the predicted molecular weight of Cadherin-16 (CDH16), with minimal or no detectable signal in mouse and rat samples under these conditions. This pattern supports kidney-restricted expression with preferential detection in human tissue.