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Home >> Antibodies >> Recombinant CAD Antibody / CALD1 / Caldesmon (HMW)

Recombinant CAD Antibody / CALD1 / Caldesmon (HMW) [clone RMCDN1-1] (V7200)

  Catalog No Formulation Size Price (USD)  
Image V7200-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7200-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7200SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7200IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recrabbitmono
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IHC testing of FFPE human uterus with recombinant CAD antibody (clone RMCDN1-1). HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name RMCDN1-1
Purity Protein A affinity chromatography
UniProt Q05682
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This recombinant CAD antibody is available for research use only.
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Description

Caldesmon is an actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity). [UniProt]

Application Notes

Titering of the recombinant CAD antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A full length human protein was used as the immunogen for the recombinant CAD antibody.

Storage

Store the recombinant CAD antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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