CA9 Antibody / Carbonic Anhydrase IX [clone CA9/3406] (V8463)

Western blot testing of human HEK293 cell lysate with CA9 antibody. Predicted molecular weight: 50-55 kDa.

Flow cytometry testing of FPA fixed human U-87 MG cells with CA9 antibody; Red=isotype control, Blue= CA9 antibody.

IHC staining of FFPE human kidney with CA9 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney with CA9 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CA9 antibody. These results demonstrate the foremost specificity of the CA9/3406 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CA9 antibody as confirmation of integrity and purity.