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Home >> Antibodies >> CA9 Antibody / Carbonic Anhydrase IX

CA9 Antibody / Carbonic Anhydrase IX [clone CA9/3406] (V8463)

  Catalog No Formulation Size Price (USD)  
Image V8463-100UG 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 429
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V8463-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V8463SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
Western blot testing of human HEK293 cell lysate with CA9 antibody. Predicted molecular weight: 50-55 kDa.
Flow cytometry testing of FPA fixed human U-87 MG cells with CA9 antibody; Red=isotype control, Blue= CA9 antibody.
IHC staining of FFPE human kidney with CA9 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney with CA9 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using CA9 antibody. These results demonstrate the foremost specificity of the CA9/3406 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CA9 antibody as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name CA9/3406
Purity Protein G affinity chromatography
UniProt Q16790
Localization Cell surface, cytoplasmic
Applications Flow cytometry : 1-2ug/million cells
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This CA9 antibody is available for research use only.
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Description

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowmans capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Application Notes

Optimal dilution of the CA9 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 314-410 from the human protein was used as the immunogen for the CA9 antibody.

Storage

Store the CA9 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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