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Home >> Antibodies >> C4d Antibody Complement 4d

C4d Antibody Complement 4d [clone CPT4d-1] (V7025)

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  Catalog No Formulation Size Price (USD)  
Image V7025-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7025-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7025SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human kidney transplant tissue stained with C4d antibody (clone CPT4d-1). Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human kidney transplant tissue stained with C4d antibody (clone CPT4d-1). Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 min.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CPT4d-1
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 720 , 721 (Human)
Localization Intracytoplasmic vacuoles of endothelial cells, Secreted
Applications Immunohistochemistry (FFPE) : 1:200-1:400 for 30 min at RT
Limitations This C4d antibody is available for research use only.
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Description

This antibody is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound protein. C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of antibodies to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product C4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. C4d has been shown to be a significant predictor of transplant kidney graft survival. C4d antibody, combined with antibody to C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the C4d antibody to be titered up or down for optimal performance.

Immunogen

Human protein was used as the immunogen for this C4d antibody.

Storage

Store the C4d antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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