- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
C-terminal-binding protein 2 Antibody / CTBP2 [clone 7F3E1] (RQ7571)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ7571 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 439 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2a |
| Clone Name | 7F3E1 |
| Purity | Antigen affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | P56545 |
| Localization | Nucleus, Cell junction |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Flow Cytometry : 1-3ug/million cells Immunofluorescence : 5ug/ml |
| Limitations | This C-terminal-binding protein 2 antibody is available for research use only. |
|
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C-terminal-binding protein 2 antibody detects CTBP2, a transcriptional coregulator that integrates metabolic status, chromatin structure, and gene expression programs across diverse tissues. The UniProt recommended name is C-terminal-binding protein 2 (CTBP2). CTBP2 belongs to the CtBP family of NAD-dependent transcriptional regulators that function as sensors of cellular redox state. By responding to intracellular NAD levels, CTBP2 links metabolic conditions to transcriptional repression or activation, influencing development, differentiation, proliferation, and stress adaptation.
C-terminal-binding protein 2 antibody identifies multiple isoforms generated through alternative promoter usage and splicing. A well-known variant, often referred to as the RIBEYE isoform, is expressed in sensory tissues and plays a structural role in ribbon synapses of photoreceptors and hair cells. Other CTBP2 isoforms localize predominantly to the nucleus and act as transcriptional corepressors. These variants share conserved protein interaction motifs that recruit chromatin modifying enzymes, histone deacetylases, and additional transcriptional regulators. Through these complexes, CTBP2 modulates chromatin accessibility and gene expression landscapes associated with cell identity and environmental adaptation.
The CTBP2 gene is located on chromosome 10q26.13 and exhibits widespread expression across tissues, including brain, endocrine organs, epithelium, immune cells, and developing tissues. Nuclear isoforms of CTBP2 interact with sequence specific transcription factors and coregulators to repress genes involved in apoptosis, epithelial to mesenchymal transition, and metabolic pathways. CTBP2 also binds NAD, using it as a cofactor that stabilizes its protein conformation. This biochemical feature enables CTBP2 to act as a redox sensor, altering its transcriptional activity when cellular NAD levels shift during metabolic stress, proliferation, or differentiation.
Structurally, CTBP2 contains a conserved dehydrogenase-like domain that binds NAD, along with protein interaction regions mediating cooperative dimerization and recruitment of chromatin modifying enzymes. Nuclear isoforms function primarily as corepressors, assembling multiprotein complexes that regulate diverse gene networks controlling cell cycle progression, differentiation, and tissue remodeling. The RIBEYE variant, in contrast, contains an extended N-terminal domain critical for forming ribbon synapses in the retina and cochlea. These synaptic ribbons support sustained neurotransmitter release required for high fidelity sensory transmission. Because of this specialized role, CTBP2 is frequently used as a marker for ribbon synapse structure in retinal and auditory neuroscience.
CTBP2 integrates into numerous developmental and physiological pathways. During embryogenesis, it regulates differentiation of epithelial, neuronal, and mesoderm derived lineages by shaping gene expression programs responsive to growth factors and metabolic cues. In adult tissues, CTBP2 participates in renewal and repair processes by modulating transcriptional networks linked to stemness, cellular polarity, and extracellular matrix interactions. Its NAD sensitive regulatory mechanism allows CTBP2 to link energy metabolism to long term changes in gene transcription, influencing how cells adapt to nutrient availability, oxidative stress, and proliferative signals.
Pathologically, dysregulated CTBP2 expression or activity has been associated with cancer, neurodegeneration, metabolic disease, and developmental abnormalities. Elevated CTBP2 levels contribute to tumor progression in several cancers by repressing epithelial determinants, enhancing invasive behavior, and modifying cell survival pathways. CTBP2 can promote oncogenic transcriptional programs when excessively activated or when recruited by oncogenic transcription factors. In neurological disease, disruptions of the RIBEYE isoform impair ribbon synapse architecture, contributing to sensory deficits in hearing and vision. Altered CTBP2 activity has also been implicated in metabolic dysfunction where redox imbalance affects transcriptional control of stress response pathways.
Because CTBP2 sits at the interface of metabolism, chromatin regulation, and transcriptional control, it continues to attract interest in studies of cell identity, metabolic adaptation, and disease progression. The ability of CTBP2 to assemble large repressive complexes makes it an important determinant of epigenetic landscapes that govern proliferation, differentiation, and tissue remodeling. Its RIBEYE isoform further positions CTBP2 as a key structural component of sensory neurotransmission, linking genetic regulation to synaptic physiology.
C-terminal-binding protein 2 antibody is validated for use in relevant research applications to detect C-terminal-binding protein 2 expression and investigate transcriptional control, metabolic signaling, sensory synapse biology, and chromatin regulation. NSJ Bioreagents provides C-terminal-binding protein 2 antibody reagents optimized for studies in epigenetics, neuroscience, developmental biology, oncology, and metabolic research.
Optimal dilution of the C-terminal-binding protein 2 antibody should be determined by the researcher.
E. coli-derived recombinant human protein (amino acids H321-Q445) was used as the immunogen for the C-terminal-binding protein 2 antibody.
After reconstitution, the C-terminal-binding protein 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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