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Home >> Antibodies >> c-Src Antibody

c-Src Antibody (F50715)

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  Catalog No Formulation Size Price (USD)  
Image F50715-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50715-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of lysate from (1) human MCF-7, (2) rat PC-12, and (3) mouse C2C12 cell line using c-Src antibody at 1:1000.
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Western blot analysis of lysate from (1) human MCF-7, (2) rat PC-12, and (3) mouse C2C12 cell line using c-Src antibody at 1:1000.
IHC analysis of FFPE human breast carcinoma tissue stained with the c-Src antibody
Confocal immunofluorescent analysis of c-Src antibody with A375 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red).
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P12931
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Immunofluorescence : 1:10-1:50
Limitations This c-Src antibody is available for research use only.
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Description

The gene for Src is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. It is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in Src could be involved in the malignant progression of colon cancer.

Application Notes

Titration of the c-Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for this c-Src antibody.

Storage

Aliquot the c-Src antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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