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Home >> Antibodies >> c-Raf Antibody / Raf1

c-Raf Antibody / Raf1 (F54689)

  Catalog No Formulation Size Price (USD)  
Image F54689-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54689-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE mouse skeletal muscle tissue with c-Raf antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse Neuro-2a cell lysate with c-Raf antibody. Predicted molecular weight ~73 kDa.
Western blot testing of human Jurkat cell lysate with c-Raf antibody. Predicted molecular weight ~73 kDa.
Flow cytometry testing of mouse Neuro-2a cells with c-Raf antibody; Left=isotype control, Right= c-Raf antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
UniProt Q99N57
Localization Cytoplasmic, nuclear
Applications Immunohistochemistry (FFPE) : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Western blot : 1:500-1:2000
Limitations This c-Raf antibody is available for research use only.
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Description

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3 (By similarity).

Application Notes

The stated application concentrations are suggested starting points. Titration of the c-Raf antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 86-113 from the mouse protein was used as the immunogen for the c-Raf antibody.

Storage

Aliquot the c-Raf antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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