KIT Antibody / CD117 Polyclonal Antibody (R31671)

KIT Antibody in HepG2 Cells WB. Western blot testing of HepG2 cell lysate with KIT Antibody. A strong immunoreactive band is detected in the approximately 120-145 kDa region, consistent with the precursor and mature forms of KIT/CD117. The higher apparent molecular weight of mature KIT reflects extensive receptor glycosylation during processing and trafficking to the cell surface. The discrete target-associated signal with minimal additional reactivity demonstrates clean detection of endogenous KIT and supports western blot use of the CD117 Polyclonal Antibody.

KIT Antibody Recombinant Protein WB. Western blot testing of 0.5 ng recombinant human KIT protein with KIT Antibody. A strong, discrete band is detected at approximately 50 kDa, corresponding to the recombinant KIT protein preparation used in the assay. The concentrated target signal and absence of appreciable additional bands demonstrate direct recognition of KIT-derived protein and provide complementary western blot validation for the CD117 Polyclonal Antibody.

KIT Antibody in Human Intestinal Cancer IHC. Immunohistochemistry staining of FFPE human intestinal cancer tissue with KIT Antibody. Distinct brown immunoreactivity is visible within the tissue, with particularly prominent staining in stromal and spindle-shaped cellular regions surrounding tumor structures. The staining is clearly distinguishable from the blue hematoxylin-counterstained nuclei and demonstrates detection of endogenous KIT/CD117 in human tumor tissue, supporting IHC use of the CD117 Polyclonal Antibody.

KIT Antibody in K562 Cells FACS. Flow cytometry analysis of human K562 cells with KIT Antibody at 1 ug/10^6 cells following blocking with goat serum. Cells alone are shown in red, the isotype control in green and KIT Antibody staining in blue. The pronounced rightward shift of the antibody-stained population relative to both controls demonstrates strong detection of cell-surface KIT/CD117 on K562 cells and supports flow cytometry use of the CD117 Polyclonal Antibody.