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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
BUB1 Antibody / Mitotic Checkpoint Kinase Antibody (RQ6759)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ6759 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | O43683 |
| Localization | Nuclear, cytoplasmic |
| Applications | Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Direct ELISA : 0.1-0.5ug/ml |
| Limitations | This BUB1 Antibody / Mitotic Checkpoint Kinase Antibody is available for research use only. |
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BUB1 Antibody recognizes BUB1, a serine/threonine protein kinase that plays a central role in the spindle assembly checkpoint (SAC) during mitosis. BUB1 helps ensure accurate chromosome segregation by coordinating checkpoint signaling at kinetochores and delaying anaphase until chromosomes are properly attached to spindle microtubules. The protein belongs to a conserved family of mitotic checkpoint regulators originally identified through studies of budding uninhibited by benzimidazoles. The Mitotic Checkpoint Kinase Antibody targets a protein involved in maintaining chromosomal stability during cell division.
The human BUB1 gene is located on chromosome 2q13 and encodes a protein of approximately 1,085 amino acids with a predicted molecular weight near 122 kDa. BUB1 contains an N-terminal region involved in kinetochore localization, regulatory domains that mediate interactions with checkpoint proteins, and a C-terminal serine/threonine kinase domain. Its recruitment to kinetochores depends on interactions with proteins including BUB3 and components of the KNL1 signaling platform. These interactions position BUB1 within the molecular machinery responsible for monitoring chromosome attachment.
BUB1 contributes to spindle checkpoint signaling by supporting the recruitment and organization of proteins involved in preventing premature anaphase onset. It also phosphorylates histone H2A at threonine 120, helping recruit shugoshin proteins that protect centromeric cohesion and support chromosome alignment. Through these activities, BUB1 coordinates kinetochore signaling, centromere organization, and chromosome segregation. The Mitotic Checkpoint Kinase Antibody is relevant to investigations of mitotic regulation and chromosome stability.
Abnormal BUB1 expression or activity can disrupt mitotic checkpoint function and contribute to chromosomal instability, a characteristic frequently observed in cancer. Altered BUB1 signaling has been investigated in multiple malignancies, although its biological effects depend on cellular context and the nature of checkpoint disruption. BUB1 is also studied in relation to aneuploidy, cell cycle progression, and the response of dividing cells to spindle damage. Understanding its regulatory functions provides insight into mechanisms that maintain genomic integrity.
Research involving BUB1 contributes to understanding kinetochore function, spindle assembly checkpoint signaling, chromosome segregation, and cancer-associated chromosomal instability. Its interactions with other mitotic regulators make it an important component of cell division research. NSJ Bioreagents offers antibodies targeting proteins involved in cell cycle control and signal transduction. A BUB1 Antibody provides a research tool for examining mitotic checkpoint regulation, kinetochore signaling, and mechanisms responsible for accurate chromosome segregation.
Explore our Signal Transduction Antibodies collection for additional antibodies targeting protein kinases, regulatory signaling proteins, and factors involved in cell cycle control.
Optimal dilution of the BUB1 Antibody / Mitotic Checkpoint Kinase Antibody should be determined by the researcher.
Recombinant human protein (amino acids E26-K1085) was used as the immunogen for the BUB1 antibody.
After reconstitution, the BUB1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
BUB1 Mitotic Checkpoint Serine Threonine Kinase antibody, BUB1B Homolog 1 antibody, Budding Uninhibited by Benzimidazoles 1 antibody, BUB1 Kinase antibody, Spindle Assembly Checkpoint Kinase antibody, BUB1 Protein Kinase antibody
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