BRG1 Antibody / SMARCA4 [clone BRG1/7633R] (V4587)

BRG1 Antibody / SMARCA4 (clone BRG1/7633R). Immunohistochemistry analysis of BRG1 / SMARCA4 in FFPE human ovarian small cell carcinoma tissue demonstrates predominantly negative tumor cell nuclei with only scattered HRP-DAB brown nuclear staining in a small subset of cells. This staining pattern is consistent with the known loss or reduced expression of BRG1 in many SMARCA4-deficient ovarian small cell carcinomas. Occasional positive nuclei may represent residual BRG1-expressing cells or non-tumor stromal components within the tumor microenvironment. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris buffer with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

BRG1 Antibody / SMARCA4 (clone BRG1/7633R). Immunohistochemistry analysis of BRG1 / SMARCA4 in FFPE human colon tissue shows strong HRP-DAB brown nuclear staining in epithelial cells lining the colonic glands, consistent with the nuclear localization of the BRG1 chromatin remodeling ATPase within the SWI/SNF complex. The staining highlights nuclei of glandular epithelial cells while surrounding stromal cells show comparatively weaker nuclear signal. The inset negative control, in which PBS was used in place of the primary antibody, shows no specific brown chromogenic staining, confirming staining specificity. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris buffer with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.