- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
BRG1 Antibody for FACS / SMARCA4 Flow Cytometry Antibody [clone 3F4] (RQ5493)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ5493 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1 |
| Clone Name | 3F4 |
| Purity | Affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide |
| UniProt | P51532 |
| Localization | Nuclear |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml Flow Cytometry : 1-3ug/million cells |
| Limitations | This BRG1 antibody is available for research use only. |
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BRG1 (SMARCA4) is an ATP-dependent chromatin remodeling enzyme that functions as the catalytic subunit of the SWI/SNF chromatin remodeling complex and regulates transcription through nucleosome repositioning and chromatin accessibility. BRG1 Antibody for FACS / SMARCA4 Flow Cytometry Antibody (clone 3F4) is a mouse monoclonal antibody developed for flow cytometry analysis of intracellular BRG1 protein expression. BRG1, also known as Brahma related gene 1, is encoded by the SMARCA4 gene and localizes primarily to the cell nucleus where it regulates transcriptional programs involved in cellular differentiation, proliferation, and tumor suppression. Because BRG1 is an intracellular nuclear protein, flow cytometry analysis using BRG1 Antibody for FACS is typically performed on fixed and permeabilized cells to allow antibody access to the intracellular SMARCA4 protein.
Flow cytometry analysis using BRG1 Antibody for FACS enables quantitative measurement of BRG1 expression at the single-cell level across heterogeneous cell populations. In a typical FACS workflow, cells are fixed, permeabilized, and stained with the BRG1 antibody followed by a fluorescently labeled secondary antibody to allow detection of SMARCA4 expression by fluorescence intensity. This approach allows researchers to analyze BRG1-positive and BRG1-negative cell populations using flow cytometry instruments and to quantify intracellular BRG1 protein levels within thousands of cells per second. Flow cytometry detection of SMARCA4 is particularly useful when studying chromatin remodeling proteins across mixed cell populations or when comparing BRG1 expression between experimental conditions.
FACS-based detection of BRG1 provides advantages for studies examining chromatin remodeling pathways because flow cytometry allows rapid analysis of protein expression across large cell populations while preserving single-cell resolution. BRG1 Antibody for FACS can be used to evaluate intracellular SMARCA4 expression within defined cell subsets, enabling analysis of BRG1 protein distribution during cellular differentiation, transformation, or experimental perturbation of chromatin remodeling pathways. Flow cytometry analysis can also be used to identify BRG1-expressing cell populations, compare fluorescence intensity distributions, and monitor changes in SMARCA4 expression following genetic or pharmacologic manipulation.
BRG1 functions as a central regulator of chromatin remodeling within the SWI/SNF complex and participates in gene regulation pathways that control cell cycle progression, differentiation, and DNA repair. Alterations in SMARCA4 expression have been reported in multiple tumor types, and BRG1 loss or mutation has been linked to cancers including lung carcinoma, thoracic sarcoma, and ovarian small cell carcinoma of hypercalcemic type. Flow cytometry detection of BRG1 expression therefore provides a useful experimental approach for investigating SMARCA4 protein levels in cancer cell models and for studying chromatin remodeling defects associated with tumor development.
Clone 3F4 is a mouse monoclonal antibody designed to recognize the BRG1 protein and support reproducible intracellular flow cytometry analysis. Monoclonal antibodies provide epitope-specific binding and consistent staining performance, making them well suited for flow cytometry experiments requiring reproducible fluorescence signal detection. BRG1 Antibody for FACS (clone 3F4) therefore provides a research reagent suitable for intracellular flow cytometry analysis of SMARCA4 protein expression in studies focused on chromatin remodeling biology, transcriptional regulation, and SWI/SNF complex function.
Optimal dilution of the BRG1 Antibody for FACS should be determined by the researcher.
A human recombinant protein (amino acids Q555-E763) was used as the immunogen for the BRG1 Antibody for FACS.
After reconstitution, the BRG1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
SMARCA4 antibody, BRG1 antibody, Brahma related gene 1 antibody, SWI/SNF chromatin remodeling protein antibody
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