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Home >> Antibodies >> BRD4 Antibody

BRD4 Antibody (RQ6454)

  Catalog No Formulation Size Price (USD)  
Image RQ6454 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human 293T cells with BRD4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= BRD4 antibody.
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with BRD4 antibody. Predicted molecular weight ~156 kDa (long form), but has been observed at 200+ kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O60885
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This BRD4 antibody is available for research use only.
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Description

Bromodomain-containing protein 4 is a protein that in humans is encoded by the BRD4 gene. The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.

Application Notes

Optimal dilution of the BRD4 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids A802-R1163) was used at the immunogen for the BRD4 antibody. The antibody is not suitable for the detection of the short form of the protein.

Storage

After reconstitution, the BRD4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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