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Home >> Antibodies >> Brd4 Antibody

Brd4 Antibody [clone DBH-2] (RQ4766)

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Image RQ4766 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Western blot testing of human HeLa cell lysate with Brd4 antibody. Predicted molecular weight ~156 kDa (long form, can be observed at 200+ kDa) and ~81 kDa (short form).
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Western blot testing of human HeLa cell lysate with Brd4 antibody. Predicted molecular weight ~156 kDa (long form, can be observed at 200+ kDa) and ~81 kDa (short form).
IHC staining of FFPE human kidney tissue with Brd4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with Brd4 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name DBH-2
Purity Affinity purified
UniProt O60885
Localization Nuclear
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Immunofluorescence/Immunocytochemistry : 1:50-1:200
Limitations This Brd4 antibody is available for research use only.
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Description

Bromodomain-containing protein 4 is a chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation.

Application Notes

Optimal dilution of the Brd4 antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human Brd4 was used as the immunogen for the Brd4 antibody.

Storage

Store the Brd4 antibody at -20oC.

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