BRD4 Antibody / Bromodomain-containing protein 4 (RQ6454)

Description

BRD4 is a member of the BET family of chromatin readers that bind acetyl-lysine marks on histone tails via 2 bromodomains and an extra-terminal domain. By recruiting and stabilizing P-TEFb (CDK9-Cyclin T1) at promoters and enhancers, BRD4 drives RNA polymerase II pause release and productive elongation, integrating signaling cues with rapid transcriptional programs in development, immune activation, and stress responses. The BRD4 antibody supports these studies by enabling precise detection of endogenous BRD4 in relevant models.

Dysregulated BRD4 activity contributes to oncogenic transcription, super-enhancer addiction, inflammation, and therapy resistance. Aberrant occupancy of BRD4 at lineage-defining regulatory elements sustains high-output expression of survival and proliferation genes across multiple cancer types. BRD4 has also been linked to DNA damage responses and chromatin organization, positioning it as a central node in epigenetic control and a target for small-molecule inhibitors.

The BRD4 antibody is widely used in western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and chromatin-focused assays to quantify protein abundance, map subcellular and chromatin-associated localization, and monitor pathway modulation under genetic or pharmacologic perturbation. With high specificity and consistent performance, the BRD4 antibody enables rigorous analysis of transcriptional elongation mechanisms, epigenetic regulation, and disease biology.

Application Notes

Optimal dilution of the BRD4 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids A802-R1163) was used at the immunogen for the BRD4 antibody. The antibody is not suitable for the detection of the short form of the protein.

Storage

After reconstitution, the BRD4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.