BRCA1 Antibody / Hereditary Breast Cancer DNA Repair Marker [clone BRCA1/1398] (V3240)

BRCA1 Antibody Breast Carcinoma IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue using BRCA1 antibody clone BRCA1/1398. Tumor epithelial cells demonstrate predominantly nuclear HRP-DAB brown staining consistent with the chromatin-associated localization of BRCA1 in hereditary breast cancer-associated DNA repair, homologous recombination signaling, and genomic stability maintenance pathways, while surrounding stromal regions show comparatively lower signal intensity. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling tissue sections in Tris-EDTA buffer, pH9.

BRCA1 Antibody Protein Array Validation. Analysis of a HuProt protein microarray containing more than 19,000 full-length human proteins using BRCA1 antibody clone BRCA1/1398. The antibody demonstrates highly selective recognition of BRCA1 with a Z-score of 117.17 and an S-score of 109.36, supporting exceptionally strong target specificity for this hereditary breast cancer-associated DNA repair protein relative to other proteins represented on the array. CD71b and TTC14 were identified as substantially lower-ranking signals. Z-scores represent signal intensity above array background in standard deviation units, while S-scores reflect the specificity gap between the top-ranked target and subsequent proteins. An S-score greater than 2.5 is generally considered supportive of target-specific antibody recognition.

BRCA1 Antibody Purity SDS-PAGE. SDS-PAGE analysis of purified BRCA1 antibody clone BRCA1/1398 demonstrating antibody integrity and purity under non-reducing (NR) and reducing (R) conditions. Under non-reducing conditions, the antibody migrates predominantly as an intact immunoglobulin species near approximately 150 kDa. Under reducing conditions, characteristic heavy and light chain bands are observed near approximately 50 kDa and 25 kDa, respectively, consistent with expected monoclonal antibody structure following disulfide bond reduction.