- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Brain Creatine Kinase Antibody / CKBB [clone CKBB/8843R] (V4380)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4380-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 539 | |
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V4380-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 249 | |
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V4380SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 539 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat, Hamster, Guinea pig |
| Format | Purified |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | CKBB/8843R |
| Purity | Protein A/G affinity |
| UniProt | P12277 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western Blot : 2-4ug/ml |
| Limitations | This Brain Creatine Kinase antibody is available for research use only. |
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Brain Creatine kinase antibody detects Creatine kinase B-type, also known as CKBB or brain-type creatine kinase, an ATP-regenerating enzyme that sustains cellular energy homeostasis in neurons and other tissues with high metabolic demand. The UniProt recommended name is Creatine kinase B-type (CKB). This enzyme catalyzes the reversible transfer of phosphate between ATP and creatine, forming phosphocreatine and ADP to maintain a steady ATP supply for critical cellular processes.
In neural tissue, Brain Creatine kinase antibody recognizes the major cytosolic isoform responsible for local ATP regeneration near ion pumps, synaptic vesicles, and cytoskeletal assemblies. CKB functions within the phosphocreatine shuttle, which transfers energy from mitochondria to ATP-utilizing regions of the cell. This mechanism enables neurons to sustain ion transport through Na+/K+-ATPase, support synaptic transmission, and maintain membrane excitability under intense activity. The enzyme's high turnover rate allows rapid adaptation to fluctuating energy needs during neuronal signaling.
The CKB gene, located on chromosome 14q32.33, encodes a 381-amino-acid enzyme that operates as a homodimer. Each monomer contains conserved active-site residues essential for ATP and creatine binding, with magnesium serving as a catalytic cofactor. CKBB is particularly abundant in brain and retina but is also present in testis, kidney, and certain epithelial tissues. Elevated CKBB levels in cerebrospinal fluid or plasma are clinical indicators of neural damage, stroke, or hypoxic stress. Dysregulated CKB expression has also been associated with tumor progression, as cancer cells exploit its energy-buffering function to sustain proliferation and migration.
Brain Creatine kinase antibody is an essential tool for studies involving neuronal metabolism, oxidative stress, and energy transfer. The enzyme interacts with cytoskeletal elements, ensuring that ATP regeneration occurs precisely where energy demand is highest. Experimental inhibition or deletion of Ckb leads to diminished phosphocreatine reserves, impaired neurotransmission, and increased vulnerability to metabolic stress. These characteristics establish CKB as a fundamental component of cellular resilience and bioenergetic regulation.
Outside the central nervous system, CKB contributes to energy-dependent processes such as sperm motility, smooth muscle contraction, and photoreceptor signaling. It is regulated by redox state, phosphorylation, and transcription factors including CREB and NRF1. Through its role in maintaining intracellular energy balance, CKB supports normal physiological performance across diverse tissues. NSJ Bioreagents provides Brain Creatine kinase antibody reagents validated for research applications in metabolism, neuroscience, and cancer biology.
Optimal dilution of the Brain Creatine Kinase antibody should be determined by the researcher.
Recombinant human full-length protein was used as the immunogen for the Brain Creatine Kinase antibody.
Aliquot the Brain Creatine Kinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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