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  • Email: info@nsjbio.com
Home >> Antibodies >> BRAF Antibody

BRAF Antibody [clone CCG-2] (RQ5438)

  Catalog No Formulation Size Price (USD)  
RQ5438 Antibody in PBS with 0.02% sodium azide and 50% glycerol 100 ug 359
IHC staining of FFPE human ovarian cancer with BRAF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human 1) brain and 2) HepG2 lysate with BRAF antibody. Predicted molecular weight: 85-95 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name CCG-2
Purity Affinity purified
UniProt P15056
Localization Cytoplasmic, nuclear, cell membrane
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:50-1:200
Limitations This BRAF antibody is available for research use only.
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Description

The BRAF gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. [RefSeq]

Application Notes

Optimal dilution of the BRAF antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human B Raf was used as the immunogen for the BRAF antibody.

Storage

Store the BRAF antibody at -20oC.

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